Mouse Collagen Type IV Chemiluminescent Immunoassay Kit from Innovative Research is a highly sensitive Chemiluminescent Immunoassay for meauring Collagen Type IV in biofluid samples, such as serum, plasma and other biological fluids. Reagents for up to 96 tests. This assay recognizes Mouse COL4 in samples. No significant cross-reactivity or interference between Mouse COL4 and analogues was observed. Innovative Research's Collagen Type IV Chemiluminescent Immunoassay kit uses the Sandwich-Chemiluminescent Immunoassay principle. The micro Chemiluminescent Immunoassay plate provided in this kit has been pre-coated with an antibody specific to Mouse COL4. Standards or samples are added to the micro Chemiluminescent Immunoassay plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL4 and Avidin-Horseradish Peroxidase(HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse COL4. You can calculate the concentration of Mouse COL4 in the samples by comparing the RLU value of the samples to the standard curve. Detection Target: Collagen Type IV Reactivity: Mouse Range: 0.31 - 20 ng/mL Sensitivity: 0.19 ng/mL Storage Conditions: An unopened kit can be stored at 2-8 C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.